We previously attempted to sequence the B. vosnesenskii genome using Oxford Nanopore technology...didn't work out so good. Turns out we didn't know what we were doing! So we are trying again, thanks to Janna Fierst's intrepid grad student John Sutton and a few other students in the department who got some actual training on the thing. Things looking MUCH better so far, very good output, nice read length distribution etc! Turns out there are a few useful tricks for DNA isolation quality, etc. that aren't as important for sequencing with Illumina. Hopefully the data turn out to be useful for assembly, as we very much want to be able to align our population genetics data directly to a reference genome for our target species, as opposed to cross-species alignments against available genomes (e.g., Bombus impatiens) which works ok but has some challenges.
Lozier Lab News
Dispatches from the lab and field!